Posted: Mar 03, 2022 3:45 pm
Why did the researchers choose a cell line with such a high level of LINE-1, quas?
https://www.nature.com/articles/gim2015119
How is that not affecting the test, and how does it not have implications for any possible relevance to what happens in a healthy cell line?
Why did they even only use one single cell line?
https://www.nature.com/articles/gim2015119
Long interspersed nuclear element-1 (L1) retrotransposons are jumping genes that comprise 17% of human DNA. They utilize a ‘‘copy-and-paste’’ mechanism to propagate themselves throughout the genome via RNA intermediates, a process termed retrotransposition. L1s are active in the germ line and during embryogenesis, yet they are epigenetically suppressed in somatic cells. In cancer cells, however, L1s are aberrantly activated and may have a role in genome instability, one of the hallmarks of cancer pathogenesis.
How is that not affecting the test, and how does it not have implications for any possible relevance to what happens in a healthy cell line?
Why did they even only use one single cell line?