Posted: Sep 04, 2016 12:58 pm
Calilasseia wrote:I obtained my data direct from the Swiss-Prot database. Go here and have fun searching lots of genomes.
EDIT: Here is the BLAST search I performed on the first 60 base pairs from my above sequence.
When you perform the BLAST search and click on the identifier P26367 in the list, you're taken to this page containing information about the human Pax6 gene. Under "Protein attributes", it says:
Sequence length: 422 AA
That's 422 amino acids. You'll find my sequence matches precisely. Click on this link and you're taken to the protein sequence. Let's compare my list:
MQNSHSGVNQLGGVFVNGRP
LPDSTRQKIVELAHSGARPC
DISRILQVSNGCVSKILGRY
YETGSIRPRAIGGSKPRVAT
PEVVSKIAQYKRECPSIFAW
EIRDRLLSEGVCTNDNIPSV
SSINRVLRNLASEKQQMGAD
GMYDKLRMLNGQTGSWGTRP
GWYPGTSVPGQPTQDGCQQQ
EGGGENTNSISSNGEDSDEA
QMRLQLKRKLQRNRTSFTQE
QIEALEKEFERTHYPDVFAR
ERLAAKIDLPEARIQVWFSN
RRAKWRREEKLRNQRRQASN
TPSHIPISSSFSTSVYQPIP
QPTTPVSSFTSGSMLGLTDT
ALTNTYSALPPMPSFTMANN
LPMQPPVPSQTSSYSCMLPT
SPSVNGRSYDTYTPPHMQTH
MNSQPMGTSGTTSTGLISPG
VSVPVQVPGSEPDMSQYWPR
LQ Ochre
with theirs:
MQNSHSGVNQLGGVFVNGRP
LPDSTRQKIVELAHSGARPC
DISRILQVSN GCVSKILGRY
YETGSIRPRAIGGSKPRVAT
PEVVSKIAQYKRECPSIFAW
EIRDRLLSEGVCTNDNIPSV
SSINRVLRNLASEKQQMGAD
GMYDKLRMLNGQTGSWGTRP
GWYPGTSVPGQPTQDGCQQQ
EGGGENTNSISSNGEDSDEA
QMRLQLKRKLQRNRTSFTQE
QIEALEKEFERTHYPDVFAR
ERLAAKIDLPEARIQVWFSN
RRAKWRREEKLRNQRRQASN
TPSHIPISSSFSTSVYQPIP
QPTTPVSSFTSGSMLGRTDT
ALTNTYSALPPMPSFTMANN
LPMQPPVPSQTSSYSCMLPT
SPSVNGRSYDTYTPPHMQTH
MNSQPMGTSGTTSTGLISPG
VSVPVQVPGSEPDMSQYWPR
LQ
They don't bother with the stop codon, but I think you'll all find that the sequences are an exact match.
You honestly think I wouldn't have gone to the trouble of searching actual protein and genome databases to obtain my information? Oh ye of little faith!
There is one difference. Is it important?
The seventh row up from the bottom.
Your list........ QPTTPVSSFTSGSMLGLTDT
with theirs..... QPTTPVSSFTSGSMLGRTDT