Posted: Sep 19, 2017 5:58 pm
I see I also made another typo. The Octopus protein is called Q19AB0_OCTVU in UniPROT.
It's an estrogen receptor. And alignment between the Octopus ER, and Human ER-Beta gives this result:
Identity: 26.207%
Another alignment, this time between the Human ER-Beta and Human Glucocorticoid (SR) receptor:
Identity: 15.689%
Clearly the oestrogen receptors are more diverget from the steroid receptors, than the oestrogen receptors are from each other.
It's an estrogen receptor. And alignment between the Octopus ER, and Human ER-Beta gives this result:
Identity: 26.207%
- Code: Select all
CLUSTAL O(1.2.4) multiple sequence alignment
TR|Q19AB0|Q19AB0_OCTVU -----------------------------------MIDKQV-------TAADDIKMLSPD 18
SP|Q92731|ESR2_HUMAN MDIKNSPSSLNSPSSYNCSQSILPLEHGSIYIPSSYVDSHHEYPAMTFYSPAVMNYSIPS 60
:*.: : :: *.
TR|Q19AB0|Q19AB0_OCTVU RCAHIHGSTGHAATAASSSSGHSATTSSSTTTSSSTSSSSSSSSSSSSSSSSSSANPQTP 78
SP|Q92731|ESR2_HUMAN NVTNLEGGPGRQTTS---------------------------------------PNVLWP 81
. :::.*. *: :*: * *
TR|Q19AB0|Q19AB0_OCTVU TSTTL-------EMADMQT----AGESGGVQLATTVAGD-TALLTTDFKKKYDSVVAGSS 126
SP|Q92731|ESR2_HUMAN TPGHLSPLVVHRQLSHLYAEPQKSPWCEARSLEHTLPVNRETLKRKVSGNRCASPVTGPG 141
* * :::.: : : . . .* *: : :* . :: * *:* .
TR|Q19AB0|Q19AB0_OCTVU TGNTTRLCQVCDDNASGFHYGVWSCEGCKAFFKRSIQGPVDYVCPATNSCTIDKHRRKSC 186
SP|Q92731|ESR2_HUMAN SKRDAHFCAVCSDYASGYHYGVWSCEGCKAFFKRSIQGHNDYICPATNQCTIDKNRRKSC 201
: . :::* **.* ***:******************** **:*****.*****:*****
TR|Q19AB0|Q19AB0_OCTVU QACRLRKCYEVGMNKGSQRKERKNSSNQTKVKRSSADFSDSTV-NSTSGNQPAKSQRLSK 245
SP|Q92731|ESR2_HUMAN QACRLRKCYEVGMVKCGSRRERCGYRLVRRQRSADEQLHCAGKAKRSGGHAPRVRE---- 257
************* * ..*:** . : : :. :: : : :.*: * :
TR|Q19AB0|Q19AB0_OCTVU SSSLVEELSKNDFAV-------P-ECKLNPSIPLTKNYILQLLIQVADKDLVQLINWAKH 297
SP|Q92731|ESR2_HUMAN --LLLDALSPEQLVLTLLEAEPPHVLISRPSAPFTEASMMMSLTKLADKELVHMISWAKK 315
*:: ** :::.: * .** *:*: :: * ::***:**::*.***:
TR|Q19AB0|Q19AB0_OCTVU IPGYADLSLSDQVHLIECCWMELVLLNCAYRSMEYEGKRLAFASNLILEKHHW-EILGMT 356
SP|Q92731|ESR2_HUMAN IPGFVELSLFDQVRLLESCWMEVLMMGLMWRSIDHPGKL-IFAPDLVLDRDEGKCVEGIL 374
***:.:*** ***:*:*.****::::. :**::: ** ** :*:*::.. : *:
TR|Q19AB0|Q19AB0_OCTVU QILEQVAAVSEQLLQFGINREELLLLEATILVNAEVRRLAGFSKIDDIRQ---IILNALI 413
SP|Q92731|ESR2_HUMAN EIFDMLLATTSRFRELKLQHKEYLCVKAMILLNSSMYPLVTATQDADSSRKLAHLLNAVT 434
:*:: : *.:.:: :: ::::* * ::* **:*:.: *. :: * : :***:
TR|Q19AB0|Q19AB0_OCTVU D-------TAQKYHPDNPRHVPSALLLLSHVRQASDRSIIYLQKQKDEGHVTFCELITEM 466
SP|Q92731|ESR2_HUMAN DALVWVIAKSGISSQQQSMRLANLLMLLSHVRHASNKGMEHLLNMKCKNVVPVYDLLLEM 494
* .: :: :: . *:******:**::.: :* : * :. * . :*: **
TR|Q19AB0|Q19AB0_OCTVU LEAQNSSNDIVAPRADVIGMGT------------------ 488
SP|Q92731|ESR2_HUMAN LNAHVLRGC----KSSITGSECSPAEDSKSKEGSQNPQSQ 530
*:*: . ::.: *
Another alignment, this time between the Human ER-Beta and Human Glucocorticoid (SR) receptor:
Identity: 15.689%
Clearly the oestrogen receptors are more diverget from the steroid receptors, than the oestrogen receptors are from each other.
- Code: Select all
CLUSTAL O(1.2.4) multiple sequence alignment
SP|Q92731|ESR2_HUMAN ------------------------------------------------------------
SP|P04150|GCR_HUMAN MDSKESLTPGREENPSSVLAQERGDVMDFYKTLRGGATVKVSASSPSLAVASQSDSKQRR 60
SP|Q92731|ESR2_HUMAN ------------------------------------------------------------
SP|P04150|GCR_HUMAN LLVDFPKGSVSNAQQPDLSKAVSLSMGLYMGETETKVMGNDLGFPQQGQISLSSGETDLK 120
SP|Q92731|ESR2_HUMAN ------------------------------------------------------------
SP|P04150|GCR_HUMAN LLEESIANLNRSTSVPENPKSSASTAVSAAPTEKEFPKTHSDVSSEQQHLKGQTGTNGGN 180
SP|Q92731|ESR2_HUMAN -----------------------------------------------MDIKNSPSSLNSP 13
SP|P04150|GCR_HUMAN VKLYTTDQSTFDILQDLEFSSGSPGKETNESPWRSDLLIDENCLLSPLAGEDDSFLLEGN 240
: ::. *:.
SP|Q92731|ESR2_HUMAN SSYNCSQSILPL-------------------------------------------EHGSI 30
SP|P04150|GCR_HUMAN SNEDCKPLILPDTKPKIKDNGDLVLSSPSNVTLPQVKTEKEDFIELCTPGVIKQEKLGTV 300
*. :*. *** : *::
SP|Q92731|ESR2_HUMAN YIPSSYVDSHHEYPAMTFYSPAVMNYSIPSNVTNLEGGPGRQTTSPN-------VLWPTP 83
SP|P04150|GCR_HUMAN YCQASFPG-------ANIIGNKMSAISVH--GVSTSGGQMYHYDMNTASLSQQQDQKPIF 351
* :*: . .: . : *: .. .** : . *
SP|Q92731|ESR2_HUMAN GHLSPLVVHRQLSHLYAEPQKSPWCEARS--LEHTLPVNRE-TLKRKVSGNRCASPV--- 137
SP|P04150|GCR_HUMAN NVIPPIPV-----------GSENWNRCQGSGDDNLTSLGTLNFPGRTVFSNGYSSPSMRP 400
. : *: * .. * ..:. :: :. *.* .* :**
SP|Q92731|ESR2_HUMAN ---------TGPGSKRDAHFCAVCSDYASGYHYGVWSCEGCKAFFKRSIQGHNDYICPAT 188
SP|P04150|GCR_HUMAN DVSSPPSSSSTATTGPPPKLCLVCSDEASGCHYGVLTCGSCKVFFKRAVEGQHNYLCAGR 460
: : ::* **** *** **** :* .**.****:::*:::*:* .
SP|Q92731|ESR2_HUMAN NQCTIDKNRRKSCQACRLRKCYEVGMVKCGSRRERCGYRLVRRQRSADEQLHCAGKAKRS 248
SP|P04150|GCR_HUMAN NDCIIDKIRRKNCPACRYRKCLQAGMNLEARKTKKKIKGI-QQ--------ATTGVSQE- 510
*:* *** ***.* *** *** :.** . : :: : :: :* ::.
SP|Q92731|ESR2_HUMAN GGHAPRVREL---LLDALSPEQLVLTLLEAEPPHVLISR--PSAPFTEASMMMSLTKLAD 303
SP|P04150|GCR_HUMAN TSENPGNKTIVPATLPQLTP--TLVSLLEVIEPEVLYAGYDSSVPDSTWRIMTTLNMLGG 568
.. * : : * *:* :::***. *.** : *.* : :* :*. *..
SP|Q92731|ESR2_HUMAN KELVHMISWAKKIPGFVELSLFDQVRLLESCWMEVLMMGLMWRSIDHP--GKLIFAPDLV 361
SP|P04150|GCR_HUMAN RQVIAAVKWAKAIPGFRNLHLDDQMTLLQYSWMFLMAFALGWRSYRQSSANLLCFAPDLI 628
:::: :.*** **** :* * **: **: .** :: :.* *** : . * *****:
SP|Q92731|ESR2_HUMAN LDRDEGKCVEGILEIFDMLLATTSRFRELKLQHKEYLCVKAMILLNSSMYPLVTATQDAD 421
SP|P04150|GCR_HUMAN INEQRMT-LPCMYDQCKHMLYVSSELHRLQVSYEEYLCMKTLLLLSSVPKDGLKSQELFD 687
::.:. . : : : . :* .:*.::.*::.::****:*:::**.* :.: : *
SP|Q92731|ESR2_HUMAN SSRKLAHLLNAVTDALVWVIAKSGISSQQQSMRLANLLMLLSHVRHASNKGMEHLLNMKC 481
SP|P04150|GCR_HUMAN EIR-M-TYIKELGKA---IVKREGNSSQ-NWQRFYQLTKLLDSM----HEVVENLLNYCF 737
. * : :: : .* :: :.* *** : *: :* **. : :: :*:***
SP|Q92731|ESR2_HUMAN KNV------VPVYDLLLEMLNAHVLRGCKSSITGSECSPAEDSKSKEGSQNPQSQ 530
SP|P04150|GCR_HUMAN QTFLDKTMSIEFPEMLAEIITNQIPKYSNGNIKKLLFH----QK----------- 777
:.. : . ::* *::. :: : .:..*. .*